Education

• Ph.D in Pathology and Pathophysiology, Zhejiang University, 2022
  • Supervisor: Chong Liu
• B.S. in Biotechnology, Zhejiang University, 2017

Work experience

• Postdoctoral Scholar, University of California Los Angeles, Dec 2022 to present
  • Supervisor: Baljit Khakh

Skills

In Vivo & Ex Vivo Imaging

• Chronic cranial window preparation for cortex and deep brain regions (e.g. striatum)
• Two-photon laser scanning microscopy (2PLSM) in awake, behaving mice and acute brain slices
• Functional calcium and fluorescent protein imaging

Mouse Genetics & In Vivo Manipulations

• Design and maintenance of complex transgenic lines (up to 9 alleles per model) and large-scale colonies (>100 matings)
• Stereotaxic surgeries including viral delivery (local and retro-orbital), intracranial tumor implantation
• Chemogenetics using DREADDs (hM3Dq/hM4Di)
• Behavioral assays

Molecular & Cellular Biology

• Cloning and vector construction, cell-specific and whole tissue RNA profiling, extracellular proteomics
• Cell culture: primary neuron, glioma cells, neuron-glioma co-culture

Data Processing & Computational Analysis

• Image analysis through ImageJ, suite2P, GECIquant, AQuA2, MATLAB and custom code
• Integrating functional calcium imaging data alongside simultaneously recorded behavioral data

Honors

• 2022 Outstanding Graduates of Zhejiang Province (Top 5%)
• 2021 National Scholarship
• 2021 Award of Honor for Graduate
• 2020 Top Ten Prize for Postgraduate of Poster Day, Zhejiang University School of Basic Medical Sciences